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 | | | | | | CpG-Analysis for MGMT Gene | | | | | | | MGMT Methylation Assay - quantifies CpG sites
Boost your research activities with the new MGMT methylation assay!
Quantify up to 27 CpG sites in one trial!
MGMT (human) is located at chromosome 10 (10q26). Encoding the DNA repair protein
O-Methylguanine-DNA Methyltransferase. MGNT repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. Frequently loss of gene expression occurs via hypermethylation of CpG sites within the promoter region of the MGMT gene.
Our Service
We offer a reliable and accurate assay service for analyzing the methylation level of the promoter region of the human MGMT gene.
Get each of the consecutive CpG sites analyzed.
Get detailled quantitative information about your samples.
In an unbeatable quality.
Assay Data
The region of interest is within the promoter region of the
MGMT gene, 27 CpG sites are observed in this ROI.
Therefore the assay contains 2 parts, whether you choose
one or both belonging to your interest.
Analyzing Method
1) Genomic DNA is treated with bisulfite
2) Amplification of the region of interest
3) Methylation level of each CpG site is determined by inhouse Pyrosequencing®-Technology
4) Quality Control of bisulfite rxn and sequence context are included.
Prices
The assay consists of 2 analyzing parts. Choose one of them or both together.
Part 1 (CpG 1 to 16), Part 2 (CpG) 17 to 27), both within the promoter region.
One Part Both Parts No of samples
48,00 68,00 1 to 10
45,00 60,00 11 to 20
42,00 53,00 21 to 50
39,00 49,00 51 to 100
37,00 45,00 101 to 200
On request On request 201 and more
Prices in EUR per sample (exclusive VAT).
For more information, please contact Genaxxon at: info@genaxxon.com
Some examples of genes where methylation plays a role of gene regulation are:
p14 - p15 - p16 - RB - MLH1 - DAPK - CDH1 - MGMT - LINE-1 - BRCA1 - p53 - p73.
If you are looking for pharmakokinetic relevant genes and for possible mutations in SNPs:
Cytochrom P450-Family: 1A2, 2A6, 3A5, 2D6, 2C9, 2C19;
NAT2 -SERT - VKORC1 - MDR1 - MTHFR - UGT1A1 - TMPT - TS - DPYD.
If you are looking for further assays or in other case of questions, don't hesitate to contact Genaxxon at: info@genaxxon.com
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| | | | | | Genotyping - SNP Analysis | set-up | on request | | | | | Sonderaktion bis 31.03.2007
Bestellen Sie bis zum 31.03.2007 einen Assay mit 240 Proben, legen wir Ihnen einen iPod Shuffle (1 GB, 240 Songs) zu den Ergebnissen.
Näheres erfahren Sie unter info@genaxxon.com / 240 songs
Short Description
Pyrosequencing is the method of choice if you look for polymorphisms (point mutations on different allels), whereby even the most difficult SNPs and mutations can be analysed.
Pyrosequencing gives unambiguous genotyping results as well as some redundant sequence information beyond the SNP position, which serves as a valuable internal control, obtained for each sample. Pyrosequencing presents SNPs in the context of the surrounding sequence. This guarantees that each individual analysis is correct, without the need for other controls. Pyrosequencing has an unbeaten record of success for assaying any polymorphism or mutation. Assays can be designed with great flexibility in primer placement.
Pyrosequencing® ist die Methode der Wahl wenn sie nach Polymorphismen (Punktmutationen auf unterschiedlichen Allelen) suchen, wobei
sogar die schwierigsten SNPs und Mutationen analysiert werden können. Kurzbeschreibung
Pyrosequencing® ergibt eindeutige Genotypisisierungsergebnisse und zusätzliche Informationen um die untersuchte SNP Position herum. Diese zusätzliche Information dient als wertvolle interne Standartkontrolle jeder einzelnen Probe!
Pyrosequencing® präsentiert SNPs im Zusammenhang mit der umgebenden DNA-Sequenz. Dies garantiert eine 100%-ige Sicherheit jeder einzelnen Analyse, ohne dass zusätzliche weitere Kontrollen durch geführt werden müssen.
Pyrosequencing® zeigt unschlagbare Erfolge bei der Analyse von Polymorphismen oder Mutationen.
Für jedes Problem können wir Ihnen einen Assay etablieren. Krebsdiagnostik
Sie forschen im Bereich Krebsdiagnostik?
Sie suchen Lösungen für individuelle Therapieansätze über den Methylierungsstatus von Genen?
Ihre pharmakologische Forschung stützt sich auf einzelne oder mehrere methylierte Marker?
Ihre epigenische Forschung erfordert sowohl schnelle als auch sichere reproduzierbare Ergebnisse?
Sie suchen nach einer Analysenmethode die komplette CpG Bereiche erfasst und diese exakt quantifiziert?
Dann möchten wir Ihnen erklären, weshalb wir Ihre Vorhaben unterstützen können.
Das Produkt variocancer ist ein speziell auf Ihre Erfordernisse zugeschnittener Assay zum Nachweis von Methylierungen von DNA. Wir bieten Ihnen einen Service an, der den Methylierungsstatus Ihrer DNA genau feststellen kann: ob 1%, 10% oder 100% methylierte DNA nachgewiesen wird (angegeben für jede CpG site) - auf unsere für Sie entwickelten Tests können Sie sich verlassen.
Andere Wissenschaftler haben erkannt: Pyrosequencing®, gepaart mit unserer Erfahrung,
ist die führende Technologie zum Nachweis und zur Quantifizierung von multiplen, fortlaufenden CpG sites.
Damit bestimmen wir die Hypermethylierung der DNA auch bei Vorliegen von nur wenig veränderten
Zellen innerhalb einer Vielzahl gesunder Zellen. Mehrere Analysen ermöglichen damit auch einen
Querschnitt des Methylierungsstatus durch das Gewebe.
Please find attached a short general Pyrosequencing overview: Pyrosequencing
Please find attached some details to SNP analysis by Pyrosequencing: SNP Description
Please find attached some Pyrosequencing applications: Applications For more information, please contact Genaxxon at: info@genaxxon.com
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| | | | | | Peptid Synthesis Service | | | | | | | Genaxxon can offer an outstanding range of services in peptide chemistry
- Peptides, polypeptides, cyclopeptides, and combinatorial peptide collections
- Peptoids, oligocarbamates, retro-inverso, N-alkyl- or N-acyl-peptides and cyclic peptidomimetics
- biotinylated, lipidated, fluorophore- and spin-labelled peptides
- Amino acid scans and sizing of epitopes and hormones
- Peptide substrates and inhibitors of enzymes
- Immunogenic peptides: allele-specific B-, T-helper-, CTL-epitopes
- Peptide-functionalised surfaces
- Analysis and structure elucidation of peptides (MS, HPLC-MS, Edman sequencing, enantiomer analysis)
High quality and rapid peptide mapping required?
Order overlapping peptides from Genaxxon to reach your goals in the development of diagnostic kits, drug development and basic research.
Peptide mapping from Genaxxon:
Unrivalled expertise, highest quality, lowest price! Peptide collection e.g. 40 Euro/pentadecapeptide.
Please contact Genaxxon for your personal offer at: info@genaxxon.com.
Please download peptide-file: Peptide Synthesis
Please download short introduction into peptide synthesis: Peptide-Intro
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| | | | | | Gene Expression Analysis | | | | | | | Quantitative PCR / Expression Analysis
Genaxxon offers quantitative gene expression studies of nearly any gene, e.g. human, mouse or rat genes.
Our service covers all steps in quantitative PCR studies from the experimental strategy to final data analysis. As quantitative PCR is resource intensive and needs specific equipment, outsourcing saves scientists valuable time and high instrumentation costs.
Genaxxon offers versatile quantitative PCR services, that are tailored to meet the specific needs of our customers and that can be applied to many different problems in molecular biology research.
Gene expression analyses are typically performed using gene specific probes optimised by Applied Biosystems as Assays-on-Demand¢â. SYBR green or customized TaqMan assays are available, too.
Applications
· Gene expression analysis by absolute and relative mRNA quantification.
· Allele discrimination for the study of genetic diseases.
· Analysis of oncogene amplification by quantification of gene copy numbers in whole genomes (Oncology).
· Biodistribution analysis (Gene Therapy).
· Detection of GMOs and recombinant DNA in biotechnology products.
· Monitoring of transgenic mice.
Service includes:
Consulting
- Competent assistance in the selection of project oriented strategies.
Experimental Design
- Design of gene-specific DNA primers and TaqMan probes, design, cloning and synthesis of gene-specific DNA and RNA standards.
Sample Preparation
- Isolation and purification of DNA and RNA from tissue, cells, food, biotechnology products, etc.
Assay Development
- Optimisation of new Real-Time PCR assays reaching maximum levels of sensitivity, accuracy and reproducibility.
Routine Assay Performance
- Professional performance of optimised Real-Time PCR assays with high throughput capacity.
Data Analysis
- Complete analysis of raw data for relative or absolute mRNA and/or gene quantifications allowing clear and reliable interpretation of the final results, e.g. ratio gene of interest/house keeping gene.
Documentation
- The experimental strategy, raw data and final results will be summarised in a detailed report.
Confidentiality
- Genaxxon treats confidentiality with the highest value. Thus, we do not hand over any details of a project to a third party.
Prices:
Due to specific demands of each project, prices will be calculated individually. Please ask for a customised free quotation.
A typical price example for an analysis of 4 different targets (genes) from 25 samples is:
SybrGreen quantitative PCR Assay Price in Euro
Double analysis of a sample per target ask for price
Setup for SybrGreen assay per target ask for price
TaqMan Probe Assay
Double analysis of a sample per target ask for price
Setup for TaqMan Probe assay per target ask for price
Please download pdf-file about quantitative PCR: Quantitative PCR
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Bestellen - Testen - Überzeugt sein
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