Data Comparison between different DNA-Polymerases
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Error Rate |
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Accuracy |
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Percentage of Clones with Mutations |
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(x10(-6) +/- SD) |
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(10(6)-fold amplification |
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1 kb |
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Pfunds-polymerase |
0.55 |
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1818000 |
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1.1% |
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Pwo-polymerase |
0.59 |
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1700000 |
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1.2% |
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ReproFast polymerase |
0.65 |
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1538000 |
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1.3% |
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Taq-polymerase |
3.3 +/- 0.8 |
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303000 |
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6.6% |
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PfuUltra™ polymerase |
0.4 +/- 0.04 |
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2326000 |
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0.9% |
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Pfu, PfuTurbo® polymerase |
1.3 +/- 0.2 |
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769000 |
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2.6% |
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DeepVent® polymerase |
2.7 +/- 0.2 |
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370000 |
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5.4% |
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Vent-R® polymerase |
2.8 +/- 0.9 |
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357000 |
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5.6% |
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Platinum®, Pfx-polymerase |
3.5 +/- 1.0 |
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286000 |
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7.0% |
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Error rates were measured in recommended PCR buffer using standard cycling conditions. |
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Data for competitors enzymes are taken from Stratagene. |
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© 2003 Cold Spring Harbor Laboratory Press |
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